| Klub | Manajer | Liga | Stadion | Lokasi | Didirikan |
| Kryvbas Kryvy Rih | | | Metalurh | Kryvyi Rih | 1959 |
| Nyva Vinnytsia | Yuriy Yaroshenko | Druha Liha | Tsentralnyi | Vinnytsia | 1958 |
| Epitsentr Kamianets-Podilskyi | Sergiy Nagornyak | Perscha Liga | Tonkocheyev Stadium | Kamianets-Podilskyi | 1960 |
| Stal Kamianske | | | Metalurh Dniprodzerzhynsk | Kamianske | 1926 |
| VPK-Ahro Shevchenkivka | | Perscha Liga | Kolos Arena | Shevchenkivka | 2010 |
| FC Uzhhorod | Yaromyr Loboda | Perscha Liga | Avanhard | Uzhhorod | 2015 |
| Naftovyk-Ukrnafta | | | Naftovyk Stadium | Okhtyrka | 1980 |
| Alians Lypova Dolyna | | Perscha Liga | Yuvileynyi | Lypova Dolyna | 2016 |
| Skala Stryi | Stepan Matviiv | Druha Liha | Sokil Stadium | Stryi | 2004 |
| Metalurh Donetsk | | | Shakhtar | Donetsk | 1996 |
| FC Cherkashchyna | | Druha Liha | Tsentralnyi Stadion Cherkasy | Cherkasy | 2010 |
| SC Dnipro-1 | | | Dnipro Arena | Dnipro | 2017 |
| SC Vilkhivtsi | Oleksiy Zorin | Druha Liha | Vilkhivtsi Arena | Vilkhivtsi | 1969 |
| Balkany Zorya | | Druha Liha | Boris Tropants Stadium | Zorya | 2007 |
| FK Trostyanets | Yuriy Bakalov | Druha Liha | Stadion imeni V. Kutsa | Trostianets | 2015 |
| FC Kharkiv | | | Kharkiv Dynamo | Kharkiv | 1998 |
| Krystal Kherson | | Druha Liha | Stadion Krystal | Kherson | 1960 |
| Probiy Gorodenka | Volodymyr Kovalyuk | Druha Liha | Probiy Arena | Horodenka | 1924 |
| FC Nikopol-NPHU | | Druha Liha | Stadion Elektrometalurh-NFZ | Nikopol | 2009 |
| SC Chaika | Igor Lutsenko | Druha Liha | Kozak Arena | Petropavlivska Borshchahivka | 1976 |
| UkrAgroKom Holokivka | | | Holovkivskyy Stadium | Holokivka | 2008 |
| Druzhba Myrivka | | Druha Liha | Kolos Stadium | Myrivka | 1961 |
| FC Ternopil | | Druha Liha | Ternopil City Stadium | Ternopil | 2007 |
| Peremoha Dnipro | | Druha Liha | Olimpiyski Rezervy | Dnipro | 2017 |
| Zhemchuzhyna Odessa | | Druha Liha | Stadion Spartak | Odessa | 2016 |
| PFK Zvyagel | | Druha Liha | Stadion Avanhard | Zviahel | 2007 |
| Lokomotiv Kyiv | Vadym Lazorenko | Druha Liha | Lokomotyv Stadium | Kyiv | 1919 |
| Revera 1908 | Yaroslav Matviiv | Druha Liha | Stadion Rukh | Ivano-Frankivsk | 2020 |
| FC Odesa | | | Spartak Odesa | Odessa | 1947 |
| FC Khust | | Perscha Liga | Karpaty Stadium | Khust | 2019 |
| Hazovyk Skala Stry | | | Kniazha Arena | Lviv | 1992 |
| Kulykiv-Bilka | Bogdan Kostyk | Druha Liha | Arena Kulykiv | Kulykiv | 2008 |
| Real Pharma Odesa | | Druha Liha | Spartak Odesa | Odesa | 2000 |
| FC Kalush | | Druha Liha | Khimik | Kalush | 1947 |
| FK Kudrivka | | Druha Liha | Kudrivka Arena | Kudrivka | 1982 |
| Sudnobudivnyk Mykolaiv | | Druha Liha | Tsentralnyi Mykolaiv | Mykolaiv | 2016 |
| TSK Simferopol | | | RSC Lokomotiv | Simferopol | 2014 |
| Krymteplytsia | | | Krymteplytsia | Molodizhne | 1999 |
| FC Chernigiv | Valeriy Chornyi | Perscha Liga | Yunist Stadium | Chernihiv | 2003 |
| Dynamo 2 Kyiv | | | Dynamo Club Stadium | Kiev | 1992 |
| Rukh-2 Lviv | Ivan Fedyk | Druha Liha | Skif Stadium | Lviv | 2003 |
| Polissya-2 Zhytomyr | Shyl Gautam Kishan | Druha Liha | Tsentralnyi Stadion Zhytomyr | Zhytomyr | 1959 |
| Veres-2 Rivne | | Druha Liha | Avanhard | Rivne | 1957 |
| Nyva-2 Ternopil | Sergiy Laktionov | Druha Liha | Ternopil City Stadium | Ternopil | 1978 |
| Vorskla-2 Poltava | Iya Andrushchak | Druha Liha | Oleksiy Butovsky Vorskla Stadium | Poltava | 1955 |
| FK Oleksandria-2 | Viktor Bytsyura | Druha Liha | Nika | Oleksandria | 1991 |
| Metalist 1925-2 Kharkiv | Maksym Tsvirenko | Druha Liha | Metalist Stadium | Kharkiv | 1925 |